Journal: Annals of Clinical and Translational Neurology

Article Title: Alpha‐synuclein RT ‐ QuIC assay in gastroduodenal and skin biopsies of Parkinson disease patients

doi: 10.1002/acn3.52282

Figure Lengend Snippet: Demographic, clinical, and biomarker data in patients and controls.

Article Snippet: These methodologies, named seed amplification assays (SAA), exploit the seeding potential of misfolded αSyn in biofluid or peripheral tissues, including cerebrospinal fluid (CSF), skin, olfactory and gastro‐intestinal mucosa, and potentially serum., , , , , , , Robust SAA data have been obtained using both CSF and skin, demonstrating high specificity (>90%) and sensitivity of 85–100% in PD and Dementia with Lewy Bodies (DLB) , , , , , and 75–90% in isolated REM‐Behavior Disorder (IRBD)., , However, our understanding of the accuracy of αSyn SAA when applied to other accessible tissues and biofluids is still limited., , In this explorative study, we investigated the value of the αSyn SAA Real‐Time Quaking‐Induced Conversion (RT‐QuIC) in detecting pathology in gastric and duodenal biopsies, two matrices not yet assessed deeply in PD, and compared results to skin biopsies.

Techniques: Biomarker Discovery

Journal: International Journal of Molecular Sciences

Article Title: Seeding Aggregation Assays in Lewy Bodies Disorders: A Narrative State-of-the-Art Review

doi: 10.3390/ijms251910783

Figure Lengend Snippet: Studies on α-syn-SAAs using CSF and BH samples.

Article Snippet: Brockmann et al., 2021 [ ] , RT-QuIC , CSF , NO , sporadic PD PD GBA PD LRRK2 PD recessive * 3 sporadic DLB DLB GBA NMCs * 4 , 107/26 99/26 9/26 20/26 33/26 16/26 14/26 , According to RT-QuIC kinetic characteristics, patients with severe GBA mutations had the highest seeding activity among PD patients and the largest percentage of samples with four out of four positive replicates. Among DLB patients, 79% of those with wildtype DLB exhibited positive α-syn seeding, while 100% of those with GBA mutations did. Decreased CSF levels of proteins were associated with α-syn proteostasis and α-syn seeding activity..

Techniques: Control, Phospho-proteomics, Diagnostic Assay, Activity Assay, Sonication, Biomarker Discovery, Fluorescence